| Abstract: | Applicability of the integrated form of the Michaelis-Menten equation to kinetic analysis of transport ATPases has been shown during continuous pH-metric recording of their activity. Two values of Km for both Na, K-ATPase and Ca-ATPase have been found to be consistent with the reported data. Both values of Km for Na, K-ATPase change with temperature, i. e. at 37 degrees, 26 degrees and 15 degrees C they are as follows: Km1--21, Km2--171; Km1--3.32, Km2--47; and Km1--1,2, Km2--20 microM, respectively. This method of determination of Km and V for transport ATPases compares favourably with the previously used methods in resulting efficiency. |
