Cloning, sequencing and characterisation of a downstream region of ksdDI operon of Arthrobacter simplex. |
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| Authors: | J Dziadek M Yamashita Y Murooka |
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| Institution: | Centre for Microbiology and Virology, Polish Academy of Sciences, Lód?, Poland. |
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| Abstract: | An 8-kb region downstream of the ketosteroid dehydrogenase (ksdD)-ketosteroid isomerase (ksdI) genes of Arthrobacter simplex was cloned. The nucleotide sequence of the first 3-kb segment downstream of ksdD-ksdI operon was determined. Three open reading frames (ORFs) preceded by Shine-Dalgarno (SD) sequences have been found. Homology search revealed that the putative product encoded by ORF3 has high level of similarity with the 3-oxosteroid dehydrogenases from A. simplex and P. testosteroni (90% identity in their putative active sites). The role of ORF3 product as a FAD-containing dehydrogenase in steroid degradation pathway is discussed. |
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