毒根斑鸠菊中的一个新倍半萜的分离与鉴定

为了更好的研究毒根斑鸠菊(Vernonia cumingiana Benth)叶茎的化学成分与活性测定,利用硅胶柱层析、Sephadex LH-20等柱色谱技术对毒根斑鸠菊叶茎提取物进行了分离,并采用现代波谱学技术(¹H NMR、¹³C NMR、HMBC、HMQC等)及X-射线单晶衍射技术并根据其理化性质鉴定了七个化合物的结构,分别为vernone A(1)、α-波甾醇(2)、β-谷甾醇(3)、芹菜素(4)、柽柳素(5)、羊齿烯醇(6)、4-羟基-2-甲氧基肉桂醛(7)。化合物4~7为首次从毒根斑鸠菊中分离得到,化合物1为一个新的倍半萜类化合物。采用双层琼脂扩散法对分得的单体化合物Vernone A进行了抑菌活性测定,结果显示vernone A对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、伤寒杆菌在实验浓度范围内未表现出明显抑菌作用。

Isolation and structural identification of a new sesquiterpene from Vernonia cumingiana Benth

In order to better study the bioactive compounds in the chemical research of Vernonia cumingiana Benth,Silica gel and Sephadex LH-20 colunm chromatography were used to isolate the chemical constituents,and seven compounds were isolated and identified through spectroscopic methods(¹H NMR,¹³C NMR,HMBC,HMQC,etc.)chemical methods and X-ray diffraction methods,their structures were established as vernone A(1),α-spinasterol(2),β-sitosterol(3),apigenin(4),tamarixetin(5),fernenol(6),4-hydroxy-2-methoxycinnamaldehyde(7).Compounds 4-7 were isolated from the V.cumingiana for the first time.Compound 1 is a new sesquiterpene compound.The antibacterial activity of the isolated monomeric compound vernone A was determined by double-layer AGAR diffusion method.The results showed that Vernone A had no obvious antibacterial effect on Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Salmonella typhimurium in the experimental concentration range.