白藜芦醇通过诱导细胞自噬性死亡抑制宫颈癌的研究

白藜芦醇作为一种广泛存在于药食同源植物中的非黄酮类多酚化合物,其抗肿瘤效果受到广泛关注,但在抑制宫颈癌方面仍缺乏体内效应的实验依据.本研究通过体内实验发现白藜芦醇具有明显的抗肿瘤生长作用,组织水平LC3B、P62和Beclin-1表达改变,推测白藜芦醇可能通过促进癌细胞的过度自噬抑制宫颈癌的进展;进一步通过体外细胞实验...

Resveratrol induces autophagic death of cervical cancer cells to exert antitumor effect

Resveratrol,a non-flavonoid polyphenol compound is widely present in Medicine and food homologous plants.Its anti-tumor effects have been widely recognized,but there is no experimental data reflecting the inhibition of cervical cancer in vivo.We found that resveratrol significantly inhibited tumor growth with upregulation of expression level of LC₃B and Beclin-1,while the expression level of P₆₂ was downregulated.Thus,this suggests that resveratrol may inhibit the progress of cervical cancer by promoting excessive autophagy.Furthermore,resveratrol induced autophagy-related VMP1,Beclin-1,and LC3B protein expression and promoted the formation of autophagosomes and autophagolysosomes in vitro.Finally,it was found that resveratrol promoted excessive autophagy when combined with the activator rapamycin.It also promoted cell apoptosis when combined with the autophagy inhibitor bafilomycin A1.In conclusion,resveratrol exhibits inhibitory effects on cervical cancer during both autophagy activation and inhibition.