黄芩配伍川贝母后黄芩中主要化学成分含量变化及抗肺炎作用研究

本文旨在探究黄芩川贝母不同比例配伍后对黄芩中主要化学成分含量影响及等比配伍后抗肺炎作用。将黄芩川贝母按3∶0、3∶1、3∶2、3∶3、2∶3、1∶3、0∶3比例配伍后,水提取,高效液相色谱法测定黄芩苷、汉黄芩苷、黄芩素、汉黄芩素含量。选择健康ICR小鼠36只,随机分为6组,分别为空白组、LPS组、阳性药组、黄芩组、川贝母组、黄芩-川贝母组,每组6只,连续给药6天;第7天,实验组腹腔注射LPS 10 mg/kg,空白组注射等体积的生理盐水,LPS造模6 h,检测各组小鼠血清中IL-1β、IL-6、TNF-α水平,光镜下观察各组小鼠肺组织病理变化。结果发现黄芩川贝母配伍比例在3∶1、3∶2、3∶3、2∶3时,黄芩苷、汉黄芩苷、黄芩素、汉黄芩素总含量变化小于6%,当配伍比例达到1∶3时,四种化学成分的总量下降了23.7%。黄芩川贝母等比配伍后可显著降低小鼠血清中IL-1β、IL-6、TNF-α含量、改善肺组织病理形态,其治疗效果优于单药组。黄芩川贝母配伍比例控制在3∶1~2∶3为宜,且二者在抗肺炎中具有协同作用。

Study on the content changes of main chemical components and anti-pneumonia in Scutellariae Radix after compatibility with Fritillariae Cirrhosae Bulbus

The purpose of the study was to investigate the effect of different proportions of Scutellariae Radix(SR)and Fritillariae Cirrhosae Bulbus(FCB)on the content of main chemical components in SR and the anti-pneumonia effect of equal proportions.SR and FCB was mixed in the ratios of 3∶0,3∶1,3∶2,3∶3,2∶3,1∶3,0∶3,and then extracted with water and determined by HPLC to determine baicalin,wogonoside,baicalein and wogonin.Thirty-six healthy ICR mice were randomly divided into six groups:control group,LPS group,treatment group,SR group,FCB group,and SRFCB group,six in each group.The drug was administered for 6 consecutive days.On the 7th day,the experimental group was injected intraperitoneally with 10 mg/kg of LPS,and the control group was injected with an equal volume of normal saline.LPS was used for 6 hours to determine the levels of IL-1β,IL-6 and TNF-αin the serum of the mice.The pathological changes of lung tissue of mice in each group were observed under light microscope.The results showed that the total content of baicalin,wogonoside,baicallein,wogonin was less than 6%when the compatibility ratio were 3∶1,3∶2,3∶3 and 2∶3.When the compatibility ratio reached 1∶3,the total amount of the four chemical components decreased by 23.7%.After equal proportions compatibility of SR and FCB,the levels of IL-1β,IL-6,TNF-αin the serum of mice were significantly reduced,and the pathological morphology of lung tissue was improved,and the therapeutic effect was better than that of the single drug group.It is advisable to control the proportions compatibility of SR and FCB to 3∶1-2∶3,and they have a synergistic effect in anti-pneumonia.