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Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum |
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| Authors: | Yashin Denis V Dukhanina Elena A Kabanova Olga D Romanova Elena A Lukyanova Tamara I Tonevitskii Alexsander G Belogurov Alexey A Raynes Deborah A Sheludchenkov Anton A Gnuchev Nikolai V Guerriero Vince Georgiev Georgii P Sashchenko Lidia P |
| Affiliation: | a Institute of Gene Biology, Russian Academy of Sciences (RAS), Moscow 119344, Russia b Centre for Medical Studies, University of Oslo, Moscow 119344, Russia c Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (RAS), Moscow 119344, Russia d Moscow State University, GSP-1, Leninskie Gory, Moscow 119991, Russian Federation e M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia f Department of Animal Sciences, University of Arizona, Tucson, AZ 85721, United States g Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, United States |
| Abstract: | Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells. |
| Keywords: | HspBP1 Tag7 Tag7-Hsp70 complex Cytotoxity Human serum |
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