| 急性缺氧增强豚鼠小脑前下动脉平滑肌细胞外向电流并抑制缝隙连接 |
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| 作者姓名： | Li XZ Si JQ Zhang ZS Zhao L Li L Ma KT |
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| 作者单位： | 石河子大学医学院新疆地方与民族高发病教育部重点实验室电生理研究室;石河子大学医学院生理学教研室; |
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| 基金项目： | supported by the National Natural Science Foundation of China(No.30900490,30960417,31100829and81000411); the Doctor Foundation of the Xinjiang Production and Construction Corps of China(No.2010GC16,2010GG34); the Doctor Foundation of Shihezi University,China(No.RCZX200921); the Science and Technology Innovation Foundation of Shihezi University,China(No.ZRKX2009ZD03,ZRKK2009YB24) |
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| 摘 要： | 本文旨在研究急性缺氧对微动脉血管平滑肌细胞(vascular smooth muscle cells,VSMCs)膜电生理特性的影响。分离出豚鼠小脑前下动脉(anterior inferior cerebellar artery,AICA)段,胶原酶A消化后用显微镊去除微动脉外层结缔组织,然后给予无糖低氧的灌流液,同时应用全细胞膜片钳技术观察VSMCs膜电流的变化。结果显示:(1)当钳制电压在40mV时,急性缺氧引起一个反应幅度为(36.4±9.2)pA的外向电流,细胞静息膜电位从(33.2±1.9)mV超极化到(38.4±1.5)mV。急性缺氧电压依赖性地增强VSMCs外向电流,主要增强0～+40mV电压区间的激活电流幅度,+40mV激活电流幅度从(650±113)pA增加到(1900±197)pA。背景灌流K+通道阻断剂tetraethylammonium(TEA,1mmol/L)后,急性缺氧对VSMCs外向电流的增强作用显著减小。(2)急性缺氧使AICA上VSMCs的细胞膜电阻(R input)从(234±63)MΩ增加到(1211±201)MΩ,细胞膜电容(C input)从(279.3±83.2)p...
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| 关 键 词： | 急性缺氧 小脑前下动脉 缝隙连接 大电导钙激活钾通道 |
Acute hypoxia increases outward current and decreases gap junction of VSMCs in guinea-pig anterior inferior cerebellar artery |
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| Li XZ,Si JQ,Zhang ZS,Zhao L,Li L,Ma KT.Acute hypoxia increases outward current and decreases gap junction of VSMCs in guinea-pig anterior inferior cerebellar artery[J].Acta Physiologica Sinica,2011,63(6):533-539. |
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| Authors: | Li Xin-Zhi Si Jun-Qiang Zhang Zhong-Shuang Zhao Lei Li Li Ma Ke-Tao |
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| Institution: | LI Xin-Zhi 1,SI Jun-Qiang 1,2,ZHANG Zhong-Shuang 1,ZHAO Lei 1,LI Li 1,MA Ke-Tao 1,1 The Key Laboratory of Xinjiang Endemic and Ethnic Diseases,2 Department of Physiology,School of Medicine,Shihezi University,Shihezi 832002,China |
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| Abstract: | The aim of the present study was to investigate the effects of acute hypoxia on the electrophysiological properties of vascular smooth muscle cells (VSMCs) in arteriole.Guinea-pig anterior inferior cerebellar artery (AICA) segments were isolated,and outer layer connective tissue was removed by collagenase A digestion and microforceps.By perfusion with physical saline solution containing no glucose and low oxygen,VSMC model of acute hypoxia was established.The model was studied by whole-cell patch clamp reco... |
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| Keywords: | acute hypoxia anterior inferior cerebellar artery gap junction big conductance calcium-activated potassium channel |
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