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| 抑制聚ADP-核糖聚合酶-1活性降低HEK293/tau441细胞tau蛋白的磷酸化 | |
| 引用本文： | Chen QH,Liao XM,Wang SH. 抑制聚ADP-核糖聚合酶-1活性降低HEK293/tau441细胞tau蛋白的磷酸化[J]. 生理学报, 2011, 63(6): 511-516 |
| 作者姓名： | Chen QH Liao XM Wang SH |
| 作者单位： | 华中师范大学生命科学学院; |
| 基金项目： | supported by the National Natural Science Foundation of China(No.30800329and30700208); Research Fund for the Doctoral Program of Higher Education of China(No.200805111019) |
| 摘 要： | 为了研究聚ADP-核糖聚合酶-1 fpoly(ADP-ribose)polymerase-1,PARP-1]对微管相关蛋白tau磷酸化水平的影响,本实验分别用不同剂量(0.5,1,2,4 mmol/L)的PARP-1的抑制剂3-氨基苯甲酰胺(3-aminobenzamide,3-AB)处理稳定表达tau441蛋白的HE... |
| 关 键 词： | 聚ADP-核糖聚合酶-1 糖原合酶激酶-3 tau蛋白 阿尔茨海默病 |
Suppressing poly(ADP-ribose)polymerase-1 inhibits tau phosphorylation in HEK293/tau441 cells |
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| Chen Qin-Hao,Liao Xiao-Mei,Wang Shao-Hui. Suppressing poly(ADP-ribose)polymerase-1 inhibits tau phosphorylation in HEK293/tau441 cells[J]. Acta Physiologica Sinica, 2011, 63(6): 511-516 | |
| Authors: | Chen Qin-Hao Liao Xiao-Mei Wang Shao-Hui |
| Affiliation: | CHEN Qin-Hao,LIAO Xiao-Mei,WANG Shao-Hui College of Life Science,Huazhong Normal University,Wuhan 430079,China |
| Abstract: | The study aimed to investigate the effect of inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) activity on tau phosphorylation in HEK293/tau441 cells and its mechanism.HEK293/tau441 cells were treated with 3-aminobenzamide (3-AB),a PARP1 inhibitor,at different doses (0.5,1,2,4 mmol/L).After 24 h,the cell morphology was observed under phase contrast microscope,tau phosphorylation level in different sites (tau-1,tau-5,Thr231) and the activity of glycogen synthase kinase 3 (GSK-3) were detected by Western b... |
| Keywords: | poly(ADP-ribose)polymerase-1 glycogen synthase kinase 3 tau protein Alzheimer's disease |
| 本文献已被 CNKI PubMed 等数据库收录！ | |