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The isolation and properties of a DNA-unwinding protein from Ustilago maydis.
Authors:G R Banks  A Spanos
Affiliation:Department of Biochemistry University of Miami School of Medicine P.O. Box 520875, Miami, Fla 33152, U.S.A.
Abstract:The direction of DNA chain growth in thymine-depleted bacteria was determined by comparing the rate of release of radioactive label by Escherichia coli exonuclease I from pulse-labeled DNA chains to that of uniformly labeled DNA of the same size. Radioactive label was found to be distributed throughout the length of the pulse-labeled DNA, indicating that longer chains arise through the joining of many extremely small polynucleotide chains.
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