The isolation and properties of a DNA-unwinding protein from Ustilago maydis. |
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Authors: | G R Banks A Spanos |
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Affiliation: | Department of Biochemistry University of Miami School of Medicine P.O. Box 520875, Miami, Fla 33152, U.S.A. |
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Abstract: | The direction of DNA chain growth in thymine-depleted bacteria was determined by comparing the rate of release of radioactive label by Escherichia coli exonuclease I from pulse-labeled DNA chains to that of uniformly labeled DNA of the same size. Radioactive label was found to be distributed throughout the length of the pulse-labeled DNA, indicating that longer chains arise through the joining of many extremely small polynucleotide chains. |
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