A simple method for mass isolation of protoplasts from species of Monostroma, Enteromorpha and Ulva (Chlorophyta, Ulvales) |
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Authors: | Srinivasa Rao Uppalapati Yuji Fujita |
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Institution: | (1) Faculty of Fisheries, Nagasaki University, Bunkyo Machi 1–14, Nagasaki, 852–8521, Japan |
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Abstract: | A simple enzyme mixture containing 2% Cellulase Onozuka R–10 and1% Macerozyme R–10 prepared in deionised water supplemented with 3% NaCland 1 mM CaCl2 was developed for isolating rapidlyprotoplasts from different species of Monostroma,Enteromorpha and Ulva. The yield fordifferent species of Monostroma ranged from 9.6 ×106 to 10.2 × 106 cells g–1f. wt thallus, and forEnteromorpha from 3.48 × 106 to 11.7× 106 cells g–1 f. wt and forUlva from 4.58 × 106 to 26.8 ×106 cells g–1 f. wt. The overallregeneration rate of the protoplasts isolated was usually > 90% and showednormal morphogenesis. The method yields rapid mass production of viableprotoplasts with high regeneration rates. |
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Keywords: | Aquaculture Cellulase Chlorophyta Enteromorpha Macerozyme Monostroma Protoplast Regeneration Ulva |
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