| miR-15a 和miR-16-1 模拟物对人骨肉瘤细胞系SOSP-9607 凋亡
和增殖的影响 |
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| 引用本文： | 蔡承魁贠喆张涛姜扩高杰裘秀春马保安.miR-15a 和miR-16-1 模拟物对人骨肉瘤细胞系SOSP-9607 凋亡
和增殖的影响[J].现代生物医学进展,2011,11(8):1417-1419. |
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| 作者姓名： | 蔡承魁贠喆张涛姜扩高杰裘秀春马保安 |
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| 作者单位： | 第四军医大学唐都医院全军骨科中心暨全军骨肿瘤研究所,陕西,西安,710038 |
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| 摘 要： | 目的:探讨miR-15a和miR-16-1模拟物对于人骨肉瘤细胞系SOSP-9607凋亡和增殖的影响。方法:将SOSP-9607细胞分为实验组和对照组。实验组分为miR-15a组、miR-16-1组、miR-15a+miR-16-1组。以miR-15a组为例,采用miR-15a模拟物(hsa-miR-15a mimics)上调SOSP-9607细胞内的miR-15a表达量。对照组分为阴性对照组和空白对照组。采用流式细胞仪测定细胞凋亡率,四甲基偶氮唑蓝(MTT)法测定细胞增殖,并计算细胞增殖效率。结果:通过统计学分析,实验组凋亡率与阴性对照组凋亡率相比明显增高(P<0.05);实验组的细胞增殖率明显低于对照组(P<0.05)。结论:上调SOSP-9607细胞内miR-15a和miR-16-1的表达量可促进SOSP-9607细胞的凋亡并抑制其增殖。
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| 关 键 词： | 骨肉瘤 成骨肉瘤细胞系SOSP-9607 miR-15a miR-16-1 细胞凋亡 细胞增殖 |
Effects of miR-15a and miR-16-1 mimics on Apoptosis and Proliferation of
Human Osteosarcoma Cell Line SOSP-9607 |
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| CAI Cheng-kui,YUN Zhe,ZHANG Tao,JIANG Kuo,GAO Jie,QIU Xiu-chun,MA Bao-an.Effects of miR-15a and miR-16-1 mimics on Apoptosis and Proliferation of
Human Osteosarcoma Cell Line SOSP-9607[J].Progress in Modern Biomedicine,2011,11(8):1417-1419. |
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| Authors: | CAI Cheng-kui YUN Zhe ZHANG Tao JIANG Kuo GAO Jie QIU Xiu-chun MA Bao-an |
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| Institution: | CAI Cheng-kui,YUN Zhe,ZHANG Tao,JIANG Kuo,GAO Jie,QIU Xiu-chun,MA Bao-an(Department of Orthopedic Surgery and Orthopedic Oncology Institute of PLA,Tangdu hospital,Fourth Military Medical University,Xi' an,Shaanxi 710038,China) |
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| Abstract: | Objective: To investigate the effects of miR-15a and miR-16-1 mimics on apoptosis and proliferation of human osteosarcoma cell line SOSP-9607.Methods: Cultured SOSP-9607 cells were divided into experimental group and control group: the experimental groups contained miR-15a group,miR-16-1 group and miR-15a+miR-16-1 group.Take miR-15a group for example,we use hsa-miR-15a mimics to upgrade the expression of miR-15a in SOSP-9607 cells.The control groups contained negative control and the blank control.The apopt... |
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| Keywords: | miR-15a miR-16-1 |
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