| 从非变性聚丙烯酰胺凝胶回收纯化DNA样本 |
| |
| 引用本文： | 朱玉君,樊叶杨,庄杰云. 从非变性聚丙烯酰胺凝胶回收纯化DNA样本[J]. 生物技术通报, 2010, 0(1) |
| |
| 作者姓名： | 朱玉君 樊叶杨 庄杰云 |
| |
| 作者单位： | 中国水稻研究所,国家水稻改良中心水稻生物学国家重点实验室,杭州,310006 |
| |
| 基金项目： | 国家转基因专项基金，水稻生物学国家重点实验室自主研究课题 |
| |
| 摘 要： | 介绍一种从非变性聚丙烯酰胺凝胶中回收和纯化目标DNA片段的方法,经比较回收纯化前后PCR产物的电泳结果,表明该方法具有简单快捷和效率高的优点。
|
| 关 键 词： | 非变性聚丙烯酰胺凝胶 回收纯化 目标DNA片段 |
Rapid Recovery and Purification of DNA Fragments from Nondenaturing Polyacrylamide Gel |
| |
| Zhu Yujun,Fan Yeyang,Zhuang Jieyun. Rapid Recovery and Purification of DNA Fragments from Nondenaturing Polyacrylamide Gel[J]. Biotechnology Bulletin, 2010, 0(1) |
| |
| Authors: | Zhu Yujun Fan Yeyang Zhuang Jieyun |
| |
| Affiliation: | Zhu Yujun Fan Yeyang Zhuang Jieyun(Chinese National Center for Rice Improvement,State Key Laboratory of Rice Science,China National Rice Research Institute,Hangzhou 310006) |
| |
| Abstract: | A method of recovering and purifying DNA fragments from nondenaturing polyacrylamide gel was reported.PCR products amplified from the recovered DNA samples were detected by nondenaturing polyacylamide gel electrophoresis and compared with those from the original DNA samples isolated from rice seedlings.Results showed that this rapid method can efficiently recover the target DNA fragments from nondenaturing polyacrylamide gel. |
| |
| Keywords: | Nondenaturing polyacylamide gel Recovery and purification Target DNA fragment |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
