条锈病对小麦(Triticum aestivum L.)叶片光合功能及光合功能蛋白D1表达的影响

测定了小麦(Triticum aestivum L.)感染小麦条锈病后的光合常数,以及叶绿素含量、类囊体膜光合电子传递速率和光合反应中心D1蛋白的变化.实验显示,条锈病侵染导致感病小麦叶片净光合速率与叶绿素含量降低;抗病小麦经侵染后净光合速率却有恢复过程,叶绿素含量先降后升.此外,感病小麦叶片被侵染后全链电子传递速率受到抑制,PSII电子传递速率的变化与全链电子传递速率的变化趋势相似,但PSI电子传递速率受到的影响较小;抗病小麦小麦叶片被侵染后电子传递速率所受影响较小.同时发现,病程中,感病和抗病小麦PSII的光合反应中心D1蛋白含量变化总是与PSII电子传递速率的变化类似,推测D1蛋白的表达量变化是引起PSII电子传递活性与全链电子传递速率变化的主要因素之一.

Influence of wheat (Triticum aestivum L.) stripe rust infection on photosynthetic function and expression protein D1 of what leaves

Two wheat cultivars(Triticum aestivum L.)that differ in their sensitivities to infection(MX146:sensitive,and 88375:tolerant) were infected by Puccinia striiformis and used to evaluate the relationship between pathogen infection and host photosynthesis functions by measuring photo-synthesis constant,chlorophyll content,photosynthetic electron transport rate and expression of protein D1 of photosystem II reaction centers.We found that net photosynthetic rate and chlorophyll content decreased in the infected sensitive wheat cultivar,while both net photosynthetic rate and the chlorophyll content of the tolerant wheat has a recovery process.In infected sensitive wheat,the electron transport rate of PSII was similar to that of whole chain which was inhibited by pathogen infections,but less influence shown on electron transport rate of PSI.Whereas in infected tolerant wheat,the effects of infection on electron transport rate were minor.The protein D1 content variance always showed similar or proportional trend to that of PSII electron transport rate,indicating that suppression of D1 expression is a possible cause for the inhibition of PSII electron transport.