Roa307, a protein encoded onCoxiella burnetii plasmid QpH1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes |
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| Authors: | Zhicheng Lin Dale Howe Louis P. Mallavia |
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| Affiliation: | (1) Department of Microbiology, Washington State University, 99164-4233 Pullman, WA, USA |
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| Abstract: | TnphoA mutagenesis identified an open reading frame,roa307, immediately upstream of the partition locusqsopAB on theCoxiella burnetii plasmid QpH1. The protein sequence deduced fromroa307 displayed homology to Orf290 ofPseudomonas putida, Orf283 and Orf282 (SpoOJ) ofBacillus subtilis —hypothetical products of genes in the chromosomal replication origin region. Expression ofroa307 was demonstrated by PhoA activity of an Roa307-PhoA fusion. |
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| Keywords: | Coxiella burnetii Plasmid QpH1 Rickettsia Replication origin TnphoA |
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