Fungus fruit body lytic enzyme from a myxomycete,Badhamia utricularis |
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Authors: | Kazuo Miyairi Kazushige Konno Yukio Harada Toshikatsu Okuno |
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Institution: | (1) Laboratory of Biochemistry, Hirosaki University, 036 Hirosaki, Aomori, Japan;(2) Laboratory of Phytopathology, Faculty of Agriculture, Hirosaki University, 036 Hirosaki, Aomori, Japan |
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Abstract: | Chitinase,β-1,3-glucanase, cellulase, xylanase and protease activity were detected in a crude enzyme preparation obtained from a slime
mold (Badhamia utricularis) which was grown on autoclaved mycelia ofPholiota nameko in a petri dish. The optimal pH of the enzyme preparation for lytic activity against fruit bodies ofLentinus edodes was 4.0, and those ofβ-1,3-glucanase and cellulase were the same. On the other hand, chitinase and protease showed optimal activity at pH 5.0 and
8.0, respectively. The lytic activity was stable below 40°C but completely inactivated at 70°C, and was most stable at pH
5.0. The studies of the optimal pH, thermal stability, and pH stability, and isoelectric focusing analysis of the enzyme preparation
suggest that chitinase,β-1,3-glucanase and cellulase activities may be responsible for lysis of fruit bodies of some mushrooms. The crude enzyme preparation
from the slime mold lysed fruit bodies of several mushrooms more efficiently than did commercial lytic enzymes preparations
(Driselase and Usukizyme). |
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Keywords: | Badhamia utricularis cellulase chitinase fruit bodies lysis β -1 3-glucanase lytic enzyme myxomycete |
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