Effect of antioxidants on microscopic semen parameters,lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation |
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| Institution: | 1. Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Lalahan, Ankara, Turkey;2. Adnan Menderes University, Faculty of Veterinary Medicine, Department of Biochemistry, Aydın, Turkey;1. Regional Center of Animal Selection and Reproduction (CERSYRA), JCCM, Avenida del Vino, s/n, 13300, Valdepeñas, Spain;2. SaBio Group, National Wildlife Research Institute (IREC), CSIC-UCLM-JCCM, ETSIA, Campus Universitario s/n, 02071 Albacete, Spain;1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Aksaray University, TR68100 Aksaray, Turkey;2. Aksaray Technical Sciences Vocational School, Aksaray University, TR68100 Aksaray, Turkey;3. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey;4. Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey;5. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri, Turkey;1. Department of Veterinary Medicine, University of Sassari, Sassari, Italy;2. Centro di Competenza Biodiversità Animale, Sassari, Italy;1. Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran;2. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran |
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| Abstract: | The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora goat semen. Ejaculates collected from three Angora goat bucks, were evaluated and pooled at 37 °C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5; 5 mM) and hyaluronan (500; 1000 μl/ml), and an extender containing no antioxidants (control) were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 °C) for 20 s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control (P < 0.05) following the freeze–thawing process. The addition of 500 μl/ml hyaluronan resulted in a higher HOST percentage, compared to the addition of 1000 μl/ml hyaluronan and the control (P < 0.001). No significant difference was recorded in the percentage acrosome and total sperm abnormalities, following supplementation with antioxidants. The addition of antioxidants did not prevent malondialdehyde (MDA) formation, compared to the controls. Antioxidant treatment however decreased (P < 0.01) the superoxide dismutase (SOD) activity. The maintenance of catalase (CAT) activity was demonstrated to be insignificant following addition of antioxidants. Further studies are required to obtain more repeatable results regarding the characterization of the enzymatic and non-enzymatic antioxidant systems in cryopreserved goat sperm. |
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