| 毕赤酵母表达的neurturin对恒河猴帕金森氏病模型中黑质多巴胺能神经元的保护作用 |
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| 引用本文： | 李鸿钧,苏婷,马雁冰,和占龙,鲁帅尧,戴长柏,孙茂盛.毕赤酵母表达的neurturin对恒河猴帕金森氏病模型中黑质多巴胺能神经元的保护作用[J].生物化学与生物物理进展,2004,31(8):722-726. |
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| 作者姓名： | 李鸿钧 苏婷 马雁冰 和占龙 鲁帅尧 戴长柏 孙茂盛 |
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| 作者单位： | 中国医学科学院,医学生物学研究所,昆明,650118;中国协和医科大学,医学生物学研究所,昆明,650118 |
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| 基金项目： | 云南省自然科学基金资助项目(2003C0026Q). |
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| 摘 要： | 帕金森氏病(PD)是由于多巴胺能神经元变性、坏死，导致黑质-纹状体系统的多巴胺含量下降而引起的一种神经系统退行性疾病，目前还没有一种很好的方法能使之治愈.Neurturin（NTN）能特异地作用于中脑多巴胺能神经元，对该类神经元具营养和保护作用.经静脉注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导恒河猴产生帕金森氏病模型，并在NTN治疗组，注射MPTP之前48 h脑室内注射重组毕赤酵母表达的人NTN 1 mg. 结果表明：模型组猴均逐渐出现了PD症状，而NTN治疗组猴，PD症状比较轻或不明显；荧光分光光度法测定MPTP模型组猴黑质、壳核和尾状核多巴胺（DA）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的含量结果与正常对照组相比均显著降低，NTN治疗组猴的黑质、壳核和尾状核中的DA、5-HT和5-HIAA与对照组相比无显著性差异，而与模型组相比，DA、5-HT和5-HIAA含量均明显增加；光镜检查MPTP模型组猴黑质神经元细胞明显脱失，而NTN治疗组猴黑质神经元细胞丢失不明显，与正常对照组猴无差别.上述结果表明，制备的重组人NTN在恒河猴体内能保护中脑黑质多巴胺能神经元不受MPTP的损伤，使其DA含量及多巴胺能神经元维持正常，在MPTP存在下没有发生PD症状.
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| 关 键 词： | Neurturin，MPTP，多巴胺能神经元，多巴胺，5-羟色胺，5-羟吲哚乙酸 |
| 收稿时间： | 2/5/2004 12:00:00 AM |
| 修稿时间： | 2004/3/30 0:00:00 |
Protection of Dopaminergic Neurons by Recombinant Neurturin Secreted From Pichia pastoris in a Rhesus Monkey Model of Parkinson's Disease |
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| LI Hong-Jun,SU Ting,MA Yan-Bing,HE Zhang-Long,LU Shuai-Yao,DAI Chang-Bai and SUN Mao-Sheng.Protection of Dopaminergic Neurons by Recombinant Neurturin Secreted From Pichia pastoris in a Rhesus Monkey Model of Parkinson's Disease[J].Progress In Biochemistry and Biophysics,2004,31(8):722-726. |
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| Authors: | LI Hong-Jun SU Ting MA Yan-Bing HE Zhang-Long LU Shuai-Yao DAI Chang-Bai and SUN Mao-Sheng |
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| Institution: | Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China |
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| Abstract: | |
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| Keywords: | neurturin MPTP dopaminergic neurons dopamine 5-hydroxytrytamine 5-hydroxyindoleacetic acid |
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