The density of apical cells of dark-grown protonemata of the mossCeratodon purpureus |
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Authors: | J M Schwuchow V D Kern T Wagner F D Sack |
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Institution: | (1) Department of Plant Biology, Ohio State University, 1735 Neil Avenue, 43210 Columbus, OH, USA;(2) Present address: Institut für Biologie III, Universität Freiburg, Freiburg i. Br., Federal Republic of Germany;(3) Present address: Developmental, Cell and Molecular Biology Group, Duke University, Durham, North Carolina, USA |
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Abstract: | Summary Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the mossCeratodon purpureus. First, the volume fraction of each cell component was determined by stereology, and published values for component density were used to extrapolate to the entire cell. Second, protonemal tips were immersed in bovine serum albumin solutions of different densities, and then the equilibrium density was corrected for the mass of the cell wall. Third, apical cell protoplasts were centrifuged in low-osmolarity gradients, and values were corrected for shrinkage during protoplast isolation. Values from centrifugation (1.004 to 1.015 g/cm3) were considerably lower than from other methods (1.046 to 1.085 g/cm3). This work appears to provide the first corrected estimates of the density of any plant cell. It also documents a method for the isolation of protoplasts specifically from apical cells of protonemal filaments.Abbreviations BSA
bovine serum albumin
- ER
endoplasmic reticulum
- Vv
volume fraction |
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Keywords: | Cell density Ceratodon purpureus Protoplasts Stereology |
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