| 我国东北地区恙虫病东方体分离株基因型的研究 |
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| 引用本文： | 张志强,胡玲美,鲁志新,魏安明,李红兵,吴益民.我国东北地区恙虫病东方体分离株基因型的研究[J].微生物学杂志,2002,22(5):22-24. |
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| 作者姓名： | 张志强 胡玲美 鲁志新 魏安明 李红兵 吴益民 |
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| 作者单位： | 沈阳军区联勤部,军事医学研究所,辽宁,沈阳,110031 |
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| 摘 要： | 为了准确鉴定我国东北地区恙虫病东方体基因型 ,采用PCR ,PCR/RFLP及PCR/SSCP等技术对我国东北地区的恙虫病东方体Sta 5 6目的基因进行分析 ,并与国际参考株进行比较。结果表明 ,东北地区恙虫病东方体分离株至少存在 2种型别 ,但基因序列可能存在遗传差异。
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| 关 键 词： | 恙虫病东方体 PCR PCR/RFLP PCR/SSCP 基因型 |
| 文章编号： | 1005-7021(2002)05-0022-03 |
| 修稿时间： | 2002年1月18日 |
Determination of Gene Type of Orientia tsutsugamushi Isolated from North-East China |
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| ZHANG Zhiqiang,HU Lingmei,LU Zhixin,WEI Anming,LI Hongbing,WU Yimin.Determination of Gene Type of Orientia tsutsugamushi Isolated from North-East China[J].Journal of Microbiology,2002,22(5):22-24. |
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| Authors: | ZHANG Zhiqiang HU Lingmei LU Zhixin WEI Anming LI Hongbing WU Yimin |
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| Abstract: | In order to identify the gene type of Orientia tsutsugamushi (Ot) isolated from North-eastern China, PCR, PCR/RELP, and PCR/SSCP were used to analyze the Sta 56 gene of the Ot and made a comparison between the Ot with international reference strains. The results showed that at least two different types exists in North-eastern China, yet there might exist hereditary differences in gene sequences. |
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| Keywords: | Orientia tsutsugamushi PCR PCR/RFLP PCR/SSCP gene type |
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