Removal of Ribonucleic Acid from Bacterial Cells by Dilute Salt Solutions |
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Authors: | Masao Onisi Teiji Kato |
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Institution: |
a Department of Microbiology, Tokyo Medical and Dental University, Tokyo, Japan |
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Abstract: | Bacterial cells were impressed upon a clean glass slide, fixed in ethyl alcohol and immersed at 37°C in either of the following two salt solutions: (A) NaCl, 7.8 gm; KCl, 0.7 gm; distilled water, 1000 ml; adjusted to pH 7.0; or (B) 0.1M NaH2PO4, 400 ml; 0.1M Na2HPO4, 600 ml; KCl, 0.7 gm. After 1-5 hr soaking to remove ribonucleic acid, the slide was stained by Giemsa's method as usual. The staining revealed slender chromatinic bodies with reasonable clarity extending the whole diameter of the moderately swollen cell. The results of this method seemed to be much like those obtained after ribonuclease digestion. |
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