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Allele Distributions and Frequencies of the Six Prion Protein Gene (PRNP) Polymorphisms in Asian Native Cattle, Japanese Breeds, and Mythun (Bos frontalis) |
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| Authors: | Takeshi Shimogiri George Msalya Si Lhyam Myint Shin Okamoto Kotaro Kawabe Kazuaki Tanaka Hideyuki Mannen Mitsuru Minezawa Takao Namikawa Takashi Amano Yoshio Yamamoto Yoshizane Maeda |
| Affiliation: | 1. Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan 2. United Graduate School of Agriculture, Kagoshima University, Kagoshima, Japan 3. Frontier Science Research Center, Kagoshima University, Kagoshima, Japan 4. School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan 5. Graduate School of Agricultural Science, Kobe University, Kobe, Japan 6. Genebank, National Institute of Agrobiological Sciences, Ibaraki, Japan 7. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan 8. Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan 9. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan |
| Abstract: | Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun × cattle composite animals. At the 23-bp indel site, a deletion (23?) allele was a major allele in all populations except mythun. At the 12-bp indel site, an insertion (12+) allele was a major allele in all populations. The 14-bp indel site was polymorphic in all Asian native cattle. In the octapeptide repeat region, a six-repeat allele was a major allele in all populations, and 5/5 and 4/6 genotypes were detected in Japanese Black and Mongolian cattle and in mythun, respectively. Two nonsynonymous single nucleotide polymorphisms (SNPs) (K3T and S154N) were detected in Asian native cattle and mythun. Haplotype analysis using the genotypes of the six sites estimated 33 different haplotypes. The haplotype 23? 12? K 6 S 14+ was found in all populations. |
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