Silicon carbide fiber-mediated DNA delivery into cells of wheat (Triticum acstivum L.) mature embryos |
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Authors: | Omirulleh Serik Ismagulova Ainur Karabaev Murat Meshi Tetsno Iwabuchi Masaki |
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Institution: | (1) Department of Botany, Faculty of Science, Kyoto University, 606-01 Sakyo-Ku, Kyoto, Japan;(2) Institute of Molecular Biology and Biochemistry, 480012 Almaty, Kazakhstan |
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Abstract: | We have demonstrated that foreign DNA can be delivered into cells of mature embryos of wheat (Triticum aestivum L.) using silicon carbide fibers (SCF). The highest transient expression of thegusA (GUS) gene was detected when dry embryos were vortexed for 10–30 min in a SCF-DNA solution containing 90–120 g/l of sucrose. Up to 100 (on average 20–40) blue expression units per embryo were observed. Scutellum side and epiblast of the intact wheat embryos are preferentially transformed. When embryos with the coleoptilar tip removed were treated and allowed to germinate, GUS staining was observed in emerging leaf tissues. The potential of this new approach for stable transformation of wheat is under investigation. It has been found that callus tissues induced from the SCF treated embryos contain GUS-expressing sectors one month after treatment. |
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