Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308 |
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Authors: | Lu-Jing Ren He Huang Ai-Hua Xiao Min Lian Li-Jing Jin Xiao-Jun Ji |
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Affiliation: | (1) College of Life Science and Pharmacy, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;(2) Jiangsu Provincial Innovation Center for Industrial Biotechnology (JPICIB), No. 30 Puzhunan Road, Pukou District, 211816 Nanjing, China |
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Abstract: | Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) at different fermentation stages. According to the analysis, a regulation strategy was proposed which reinforced acetyl-CoA and NADPH supply at a specific fermentation stage. DHA content of total fatty acids was increased from 35 to 60% by the addition of 4 g/L malic acid at the rapid lipid accumulation stage. Total lipid content also showed an apparent increase of 35% and reached 19 g/L when 40 mL ethanol/L was added at the late lipid accumulation stage. |
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Keywords: | DHA Acetyl-CoA NADPH Schizochytrium sp. |
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