Fate and role of peroxisomes during the life cycle of the yeast Saccharomyces cerevisiae: inheritance of peroxisomes during meiosis |
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Authors: | Aner Gurvitz Hanspeter Rottensteiner Barbara Hamilton Helmut Ruis Andreas Hartig I W Dawes Maximilian Binder |
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Institution: | School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney 2052, Australia Tel. +612-9385-2089; fax +612-9385-1050 e-mail I.Dawes@unsw.edu.au., AU Institut für Biochemie und Molekulare Zellbiologie der Universit?t Wien und Ludwig Boltzmann Forschungstelle für Biochemie, Vienna Biocenter, A-1030 Wien, Austria, AT Institut für Tumorbiologie-Krebsforschung der Universit?t Wien, A-1090 Wien, Austria, AT
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Abstract: | Sporulation in the yeast Saccharomyces cerevisiae is a meiotic developmental process that occurs in MAT
a/MATα heterozygotes in response to nutrient deprivation. Here, the fate and role of peroxisomes during sporulation and germination
has been examined by a combination of immunoelectron microscopy and the use of pex mutants defective in peroxisomal functions. Using a green fluorescent protein probe targeted to peroxisomes we show that
peroxisomes are inherited through meiosis and that they do not increase in number either during sporulation or spore germination.
In addition, there is no requirement for peroxisome degradation prior to spore packaging. Unlike the situation in filamentous
fungi, peroxisomes do not proliferate during the yeast life cycle. Functional peroxisomes are dispensable for efficient meiotic
development on acetate medium since homozygous Δpex6 diploids sporulated well and produced mature spores that were resistant to diethyl ether. Like haploids, diploid cells can
proliferate their peroxisomes in response to oleate as sole carbon source in liquid medium, but under these conditions they
do not sporulate. On solid oleate medium, homozygous pex5,Δpex6, and pex7 cells were unable to sporulate efficiently, whereas the wild type was. The results presented here are discussed in terms
of the transmission of organelles to progeny cells.
Accepted: 19 December 1997 |
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