Biochemical and immunohistochemical studies with specific polyclonal antibodies directed against bovine myelin/oligodendrocyte glycoprotein |
| |
Authors: | Marie-Christine Birling Guy Roussel Françoise Nussbaum Jean-Louis Nussbaum |
| |
Institution: | (1) Laboratoire de Neurobiologie Ontogénique, Centre de Neurochimie du CNRS, 5, Rue Blaise Pascal, 67084 Strasbourg Cedex, France |
| |
Abstract: | Bovine myelin/oligodendrocyte glycoprotein (MOG) was purified from a Wolfgram protein fraction of brain myelin by molecular sieving and preparative gel electrophoresis. The N-terminal sequence of this wheat germ agglutinin reacting glycoprotein was determined. Antibodies against purified MOG and synthetic N-terminal octapeptide of MOG were produced in rabbits. Respective affinity purified antibody preparations gave identical results on Western blots. Treatment with specific glycosidases indicated that the oligosaccharide chains of MOG are only of N-chain type. This glycoprotein seems to be restricted to mammalian species since it was not detected in other animal species, ranging from fish up to reptiles. Immunohistochemical investigations on rat brain sections revealed that MOG is restricted to myelin sheaths and oligodendrocytes, thus corroborating previous results obtained with the MOG 8-18C5 monoclonal antibody. Decreased staining pattern in Jimpy brain further attested its specific localization in myelin-related structures. The octapeptide site-specific antibodies were not reactive on brain sections which may be attributed to the burying of this N-terminal sequence in the membrane. These MOG polyclonal antibodies appear to be valuable tools for further studies concerning this minor glycoprotein.Abbreviations BSA
bovine serum albumin
- CNS
central nervous system
- DM-20
minor myelin proteolipid protein
- MAG
Myelin-associated glycoprotein
- MBP
myelin basic proteins
- MOG
Myelin/oligodendrocyte glycoprotein
- OMgp
Oligodendrocyte/Myelin glycoprotein
- PAGE
polyacrylamide gel electrophoresis
- PBS
phosphate buffered saline
- PeptMOG
n-terminal octapeptide of MOG
- PLP
major myelin proteolipid protein
- PMSF
phenylmethylsulfonylfluoride
- SDS
sodium dodecylsulphate
- TBS
Tris buffered saline
- WPF
Wolfgram protein fraction
- WGA
Wheat germ agglutinin |
| |
Keywords: | Bovine MOG polyclonal antibodies brain development phylogenesis immunohistochemistry |
本文献已被 SpringerLink 等数据库收录! |