The preparation of the cytoplasmic and mitochondrial forms of malate dehydrogenase and aspartate aminotransferase from pig heart by a single procedure

A single procedure for the preparation of the mitochondrial and cytoplasmic forms of malate dehydrogenase and aspartate aminotransferase from pig heart is described. l-3-Hydroxyacyl CoA dehydrogenase may also be obtained from this procedure. The five enzymes are obtained in preparative amounts in homogeneous form with specific activities equal to or higher than those previously reported. These experiments also have established that the subforms of the mitochondrial isozyme of malate dehydrogenase are apparently the result of preparative manipulations.