洋葱内表皮细胞中的网状结构物

使用 Triton 抽提和卡马氏亮蓝染色方法,在洋葱鳞茎内表皮细胞中观察到网状结构物的存在。秋水仙素和细胞松弛素 B 的处理,进一步区分出两种不同的网状结构:近质膜较细的网状结构能被秋水仙素破坏;核周围与核成切线状结合的较粗的网状结构,经细胞松弛素 B 处理后,其形象更为突出。本文讨论了卡马氏亮蓝方法所揭示的带壁细胞的网状结构物。

CELLULAR NETWORKS IN EPIDERMAL PEELS OF ONION

Cellular networks in epidermal peels of onion bulb can be distinguished by first removal of superficially attached cytoplasmic constituents with Triton phos- phate buffer and then by staining with Coomassie blue R 250(Fig.4).Two distinct kinds of networks can be further recognized by treatment with colchicine and cytocha- lasins:one thinner network underneath the periphery of plasmalemma can be abolished by colchicine (Fig.7,8);and the other thicker one which associates tangentially with the nucleus was more distinctive after cytochalasin B treatment (Fig.5,6).Discussion is made regarding these two networks in wall-enclosed plant cells as revealed by the present technic.