The identification and quantitative analysis of abscisic acid in plant extracts by gas-liquid chromatography |
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Authors: | J. R. Lenton V. M. Perry P. F. Saunders |
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Affiliation: | (1) Department of Botany, University College of Wales, Aberystwyth;(2) Present address: Department of Biochemistry, University of Liverpool, Liverpool, U.K. |
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Abstract: | Summary New techniques are described which permit the quantitative analysis of microgram quantities of abscisic acid in plant extracts by gas chromatography. Presumptive methyl abscisate peaks on gas chromatograms are positively identified by photosensitised isomerisation to methyl 2-trans-abscisate. Losses of abscisic acid during pre-purification are corrected by using 2-trans-abscisic acid as an internal standard. |
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