| 鸡传染性支气管炎病毒两个地方分离株S1基因的扩增克隆与序列分析 |
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| 引用本文： | 戴亚斌 陈德胜 等. 鸡传染性支气管炎病毒两个地方分离株S1基因的扩增克隆与序列分析[J]. Virologica Sinica, 2002, 17(2): 172-177 |
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| 作者姓名： | 戴亚斌 陈德胜 等 |
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| 作者单位： | 南京农业大学动物医学院，南京农业大学动物医学院，南京农业大学动物医学院，南京农业大学动物医学院，南京农业大学动物医学院，南京农业大学动物医学院，江苏沿海地区农业科学研究所，江苏省家畜科学研究所，南京农业大学动物医学院，南京农业大学动物医学院 江苏南京210095，江苏沿海 |
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| 基金项目： | 国家自然科学基金重大项目 ( 398932 9)，江苏省自然科学基金项目 (BK99185 ) |
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| 摘 要： | 对IBV肾型毒株JS/95 /0 3和呼吸型毒株SD/97/0 1的S1全基因进行了扩增、克隆和序列测定 ,将两毒株的S1基因序列与 10个参考毒株进行了比较。结果表明 ,JS/95 /0 3和SD/97/0 1分别与M41和H12 0株亲缘关系最近 ,它们可能是疫苗毒株的变异株。JS/95 /0 3和SD/97/0 1间的亲缘关系也较近 ,两者S1蛋白中只有 2 4个氨基酸的差异 ,其中有 15个位于前 130个氨基酸中 ,第 116位氨基酸可能与毒株的致病性有关。对两毒株S1蛋白的二级结构进行了预测和比较 ,结果发现 ,一个或极少数氨基酸的差异即可导致S1蛋白二级结构和抗原性的改变
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| 关 键 词： | 传染性支气管炎病毒(IBV) S1基因 扩增 克隆 序列分析 |
Amplification, Cloning and Sequence Analysis of S1 Genes from Infectious Bronchitis Virus Isolates JS/95/03 and SD/97/01 |
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| Abstract: | The entire S1 genes from nephropathognic strain JS/95/03 and respiratory strain SD/97/01 of infectious bronchitis virus were amplified, cloned and sequenced. The nucleotide and deduced amino acid sequences of S1 genes from two isolates and ten reference strains were compared. The results showed that JS/95/03 and SD/97/01 were genetically closely related to M41 and H120, respectively. So it is speculated that they were possible variants of vaccine strains. Two isolates were different in pathogenicity, but they genetically related. Only 24 amino acid differences were found between S1 glycoproteins of two isolates, and 15 of them occurred in the region of the first 130 amino acid residues. A amino acid differece at the position 116 might be involved in pathogenicity. The comparison of the predicted secondary structures of S1 glycoproteins from two isolates revealed that only one or a few amino acid differences were responsible for different antigenicity and structure. |
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| Keywords: | Infectious bronchitis virus(IBV) S1 gene Amplification Cloning Sequence analysis |
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