Characterization of Microbial Consortia in Paddy Rice Soil by Phospholipid Analysis |
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Authors: | Q Bai A Gattinger L Zelles |
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Institution: | (1) National Agroenvironmental Protection Institute, Fukang Road No31, Tianjin, China, 300191, CN;(2) GSF-National Research Center for Environment and Health, Institute of Soil Ecology, 85764 Neuherberg, Germany, DE |
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Abstract: | Abstract
Microbial biomass and community structure in paddy rice soil during the vegetation period of rice were estimated by analysis
of their phospholipid fatty acids (PLFA), hydroxy fatty acids of lipopolysaccharides (LPS-HYFA), and phospholipid ether lipids
(PLEL) directly extracted from the soil. A clear change in the composition of the community structure at different sampling
periods was observed, indicated by the principal component analysis of the PLFA. A dramatic decline of ester-linked PLFA was
observed in the soil samples taken at the second sampling time. In contrast to the ester-linked PLFA, the non-ester-linked
PLFA composition did not change. The hydroxy fatty acids of lipopolysaccharides as well as ether lipids decreased consecutively
during the observation period. Total microbial abundance was estimated to be (4.1–7.3) × 109 cells g-1 soil (dry weight). About 44% account for aerobic and 32% for facultative anaerobic bacteria, and 24% for archaea, on average.
According to the profile and patterns of PLFA in the soil sample, it may be suggested that the paddy soil at the August sampling
period contained more abundant facultative anaerobic bacteria (ca. 36%) and archaea (ca. 37%), but the total microbial biomass
was significantly lower than in the remaining sampling periods. As the plant approached maturity, the microbial community
structure in the soil changed to contain more abundant Gram-negative bacteria and methanotrophs.
Received: 23 September 1999; Accepted: 28 February 2000; Online Publication: 12 May 2000 |
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