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| SN38对Fas介导凋亡效应的影响 | |
| 引用本文： | 曹妍,王欢,童晓鹏,单风平,梅原久.SN38对Fas介导凋亡效应的影响[J].微生物学杂志,2011,31(5):49-53. |
| 作者姓名： | 曹妍 王欢 童晓鹏 单风平 梅原久 |
| 作者单位： | 1. 中国医科大学基础医学院免疫学教研室,辽宁沈阳,110001 2. 西藏民族学院免疫学教研室,陕西成阳,712000 3. 金沢医科大学血液免疫内科,日本金沢920-0293 |
| 摘 要： | 为探讨SN38与Fas介导凋亡的相互关系，并进一步探讨脂筏（富含鞘磷脂）在该过程的作用，本实验拟单独或者联合应用SN38和CH11作用于脂筏阴性和脂筏阳性的淋巴瘤细胞株WR／Fas—SM（－）细胞和WR／Fas—SMS1细胞，通过流式细胞术、DNAladder等实验方法观察SN38能否促进Fas介导的凋亡以及对2细胞的效应差异。结果显示，SN38与CHll联合应用与单独应用SN38相比，可以诱导WR／Fas—SMS1细胞更加明显凋亡现象的发生，但对WR／Fas—SM（-）细胞凋亡的促进作用不明显，提示脂筏在SN38促进WR／Fas-SMS1细胞Fas介导凋亡过程中起到一定作用。 |
| 关 键 词： | 凋亡 SN38 脂筏 鞘磷脂 |
Influence of SN38 on Fas-Mediated Apoptosis Effect |
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| CAO Yan,WANG Huan,TONG Xiao-peng,SHAN Feng-ping,HISANORI UMEHARA,LV Chang-long.Influence of SN38 on Fas-Mediated Apoptosis Effect[J].Journal of Microbiology,2011,31(5):49-53. | |
| Authors: | CAO Yan WANG Huan TONG Xiao-peng SHAN Feng-ping HISANORI UMEHARA LV Chang-long |
| Institution: | CAO Yan1,WANG Huan1,TONG Xiao-peng2,SHAN Feng-ping1,HISANORI UMEHARA3,LV Chang-long1(1.Teach.& Res.Div of Immunol.,Coll.of Basic Med.Sci.,China Med.Uni.,Shenyang110001,2.Dept.of Immunol.,Tibet Uni.For Nationalities,Xianyang 712000,3.Dept.of Hematol.& Immunol.,Kanazawa Med.Uni.,Uchinada-machi 920-0293,Japan) |
| Abstract: | In order to investigate the mutual relationship between SN38 and Fas-mediated apoptosis and to further study the role of lipid raft(abundant in sphingomyelin) in the process,in the experiments SN38 and CH11 were worked out to react independently or jointly on lymphoma cell strains of lipid raft positive and lipid raft negative WR/Fas-SM(-) cells and WR/Fas-SMS1 cells,and observe the effect differences whether the SN38 could enhance Fas-mediated apoptosis and two cells through flow cytometry,DNA ladder and o... |
| Keywords: | apoptosis SN38 lipid raft sphingomyelin |
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