Downregulation of <Emphasis Type="Italic">AKR1B10</Emphasis> expression in colorectal cancer |
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Authors: | E S Kropotova R A Tychko O L Zinov’eva A F Zyryanova S L Khankin V L Cherkes V A Aliev S F Beresten N Yu Oparina T D Mashkova |
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Institution: | (1) Department of Biochemistry, Yamagata University School of Medicine, 2-2-2 Iidanishi, 990-9585 Yamagata, Japan;(2) Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, 565-0871 Osaka, Japan; |
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Abstract: | Colorectal cancer is one of the most common cancers in the world. Changes in AKR1B1 and AKR1B10 expression levels, whose diagnostic value was previously shown for several other cancer types, were studied in colorectal
tumors. These genes encode aldose reductases, members of the aldo-keto reductase superfamily, which comprises enzymes capable
to reduce a range of aromatic and aliphatic aldehydes and ketones. They are also involved in retinoid metabolism and carcinogenesis.
AKR1B1 and AKR1B10 mRNA levels were compared in paired specimens of normal and colorectal tumor tissues using RT-PCR and quantitative real-time
PCR. For the first time, the downregulation of these genes was demonstrated in colorectal carcinoma. AKR1B10 expression was decreased in most tumor specimens (88%, 65/74) even at the early stages, and in more than 60% of cases mRNA
levels were decreased more than 10-fold. AKR1B1 mRNA levels were decreased in 10% of specimens. Therefore, these two structurally similar genes show quite different mRNA
expression patterns in colorectal cancer, suggestive of their different functional roles in the intestine. Significant downregulation
of AKR1B10 expression can be considered a potential diagnostic marker of colorectal cancer. |
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