| 树舌多糖Fb、Fc的结构研究 |
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| 引用本文： | 梁忠岩,谭日红,张翼伸,苗春艳.树舌多糖Fb、Fc的结构研究[J].中国生物化学与分子生物学报,1994,10(4):407-412. |
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| 作者姓名： | 梁忠岩 谭日红 张翼伸 苗春艳 |
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| 作者单位： | 东北师范大学生物系 |
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| 摘 要： | 从树舌子实体中分离纯化出另两个级分Ｆｂ、Ｆｃ，经超速离心，电泳，ＳｅｐｈａｒｏｓｅＣＬ－４Ｂ柱层析等确定其均为质点、极性均一级分。经ＧＣ、ＩＯ＿４～－氧化，Ｓｍｉｔｈ降解，部分酸水解，甲基化及其产物ＧＣ，ＧＣ－ｍｓ分析，ＩＲ等分析确定Ｆｂ是由１→３甘露糖基构成主链，分枝点率及分枝率均较大；Ｆｃ由１→６和１→４甘露糖基构成主链，分枝点率及分枝率较少。
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| 关 键 词： | 树舌 甘露聚糖 |
| 收稿时间： | 1994-08-20 |
Structural Investigation of Polysaccharides of Fb and Fc from Ganoderma Applanatum |
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| Liang,Zongyan,Tan,RiHong,Zhang,Yi-shen,Miao,Chun-yan.Structural Investigation of Polysaccharides of Fb and Fc from Ganoderma Applanatum[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(4):407-412. |
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| Authors: | Liang Zongyan Tan RiHong Zhang Yi-shen Miao Chun-yan |
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| Institution: | (Depatment of Biology, Norrheast Normal University,Changchun 130024 |
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| Abstract: | Water soluble crude polysaccharide from the fruit bodies of Ganoderma Applana tum were fractionated into two fractions Fb ,Fc. They give single, symmetrical peaks on ultracentifugation,on Sepharose CL-4B and electrophoresis. Periodate oxidation, Smith degradation, partial hydrolysis, methylation analysis, IR,13C-NMR etc were empl0oyed for the structure analysis of fractions. These structure analysis indicated that Fb is a hetero polysaccharide containing a main chain of 1-3 manophyranosyl residues substituted at 0-6, and Fc containing a main chain of 1-6, 1-4 manophyranosyl residues substituted at 0-3. There are more chains in Fb than in Fc on molocule basis. |
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| Keywords: | Ganoderma Applanatum Mannan |
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