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甜叶菊中Stevioside的测定
引用本文:韦永成,.甜叶菊中Stevioside的测定[J].广西植物,1985(4):397-404.
作者姓名:韦永成  
作者单位:广西亚热带作物研究所
摘    要:在硅胶G薄层板上用氯仿:甲醇:水(38:12:2)分离出Stevioside。用蒽酮—硫酸试剂显色,然后用上海72型—分光光度计在620nm波长比色,测出甜叶菊中Stevioside的含量。本法回收率为96.50±0.539%。

关 键 词:蒽酮    薄层板    具塞刻度试管    双蒸水    薄层扫描测定    Stevioside    叶中    层离    气相色谱    标准曲线  

DETERMINATION OF STEVIOSIDE IN STEVIA REBAUDIANA BERTONI
Abstract:Stevioside can be separated on Silica gel G with chloroform-methanol - water (38: 12: 2). The areas of the spots were detected with antheron-sulfuric acid. The content of Stevioside in the leaves was determined by colorimetric method with a 72 spectrophotometer = 620nm. The recovery percentage of the method was 96.5±0.539%.
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