甜叶菊中Stevioside的测定 |
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引用本文: | 韦永成,.甜叶菊中Stevioside的测定[J].广西植物,1985(4):397-404. |
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作者姓名: | 韦永成 |
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作者单位: | 广西亚热带作物研究所 |
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摘 要: | 在硅胶G薄层板上用氯仿:甲醇:水(38:12:2)分离出Stevioside。用蒽酮—硫酸试剂显色,然后用上海72型—分光光度计在620nm波长比色,测出甜叶菊中Stevioside的含量。本法回收率为96.50±0.539%。
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关 键 词: | 蒽酮 薄层板 具塞刻度试管 双蒸水 薄层扫描测定 Stevioside 叶中 层离 气相色谱 标准曲线 |
DETERMINATION OF STEVIOSIDE IN STEVIA REBAUDIANA BERTONI |
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Abstract: | Stevioside can be separated on Silica gel G with chloroform-methanol - water (38: 12: 2). The areas of the spots were detected with antheron-sulfuric acid. The content of Stevioside in the leaves was determined by colorimetric method with a 72 spectrophotometer = 620nm. The recovery percentage of the method was 96.5±0.539%. |
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