Regulation of the maizerab17 gene promoter in transgenic heterologous systems |
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Authors: | Josep Vilardell John Mundy Bodil Stilling Bernard Leroux Maria Pla Georges Freyssinet Montserrat Pagès |
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Institution: | (1) Departmento de Genetica Molecular, Centro de Investigación y Desarrollo C.S.I.C., Jorge Girona 18-26, 08034 Barcelona, Spain;(2) Carlsberg Research Laboratory, Gl. Carlsberg Vej 10, DK-2500 Copenhagen, Denmark;(3) Service de Biologie Moléculaire et Cellulaire Végétale, Rhône-Poulenc Agrochimie, BP 9163, 69263 Lyon Cedex 09, France |
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Abstract: | The maizerab17 gene is expressed in different plant parts in response to ABA and osmotic stress (J. Vilardellet al., Plant Mol Biol 14 (1990) 423–432). Here we demonstrate that 5 upstream sequences of therab17 gene confer the appropriate patterns of expression on the chloramphenicol acetyl transferase (CAT) reporter gene in transgenic tobacco plants, as well as in protoplasts derived from cultured rice cells. Specifically, a CAT construct containing a large 5 upstream fragment ofrab17 (–1330/+29) results in high levels of CAT activity in embryos, leaves and roots of transgenic plants subjected to water stress or ABA treatment. Transient expression assays in rice protoplasts transfected with CAT genes fused torab17 promoter deletions indicate that a 300 bp DNA fragment (–351/–102) is sufficient to confer ABA responsiveness upon the reporter gene. Furthermore, a 100 bp sequence (–219/–102) is capable of conferring ABA responsiveness upon a minimal promoter derived from the 35S CaMV promoter. Gel retardation experiments indicate that maize nuclear proteins bind to this fragment. This region of 100 bp contains a sequence (ACGTGGC) which has been identified as an abscisic acid response element in studies of other ABA-responsive plant genes. |
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Keywords: | rab17 promoter abscisic acid transgene expression |
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