Cultured epithelial cells from patients with cystic fibrosis have an increased expression of the 14 kDa Ca2(+)-binding protein CFA |
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| Authors: | J Keulemans V Van Heyningen B J Scholte J Bijman A W van der Kamp M Kansen H De Jonge H Galjaard A T Hoogeveen |
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| Affiliation: | MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands. |
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| Abstract: | The Cystic Fibrosis antigen (CFA) is a 14 kDa. Ca2(+)-binding protein known to be expressed in cells of myeloid origin during normal cell differentiation. CFA serum levels are elevated in Cystic Fibrosis (CF) patients and heterozygotes. We examined the expression of CFA in different cultured epithelial cells from controls and patients with CF. The steady state level of CFA was in general higher in epithelial cells from CF patients compared to control cells and was found to increase during cell aging. The latter difference could be attributed to an increased rate of CFA synthesis rather than to an impairment of CFA degradation or secretion, as shown by pulse chase experiments. |
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