Tissue culture and micropropagation of Cuphea ericoides,a potential source of medium-chain fatty acids |
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Authors: | I Rita E I S Floh |
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Institution: | (1) CENARGEN/EMBRAPA, C.P. 02372 Brasília, 70849-970, Brasil;(2) Plant Cell Biology Laboratory, Dep. of Botany, Inst. of Biosciences, USP, C.P. 11461, 05422-970 São Paulo, Brasil |
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Abstract: | Plant rgeneration occurred on leaf-and stem-derived callus of Cuphea ericoides Cham. & Schlechtd obtained in Murashige and Skoog medium supplemented with auxins indole-3-acetic acid (IAA), -naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-d)] plus cytokinins 6-benzyladenine (BA) or kinetin]. These calluses were subcultured and showed vigorous growth. When subcultured on medium containing 2.22 or 4.44 M BA, the calluses showed profuse regeneration of shoots whereas those subcultured on medium supplemented with 2.69 M NAA or 0.226 M 2,4-d produced numerous roots. Isolated shoots rooted on Murashige and Skoog medium lacking growth regulators or containing 0.54 M NAA or 0.49 M indole-3-butyric acid (IBA). Plantlets were acclimatized to greenhouse conditions.Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige & Skoog medium
- NAA
1--naphthaleneacetic acid |
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Keywords: | propagation somaclonal variation triglycerides |
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