Uptake and Utilization of Fructose by Anabaena variabilis ATCC 29413. Effect on Respiration and Photosynthesis |
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Authors: | Valiente Eduardo Fernandez; Nieva Mar; Avendano M Carmen; Maeso Eva Sanchez |
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Institution: | Departamento de Biologia, Facultad de Ciencias, Universidad Autdnoma de Madrid 28049 Madrid, SPAIN |
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Abstract: | Anabaena variabilis ATCC 29413 showed a constitutive mechanismfor fructose uptake which was further enhanced by growing thecells with fructose. The uptake process was energydependentas indicated the inhibitory effect of the uncoupler carbonylcyanidem-chlorophenylhydrazone (CCCP) and the reduction induced byincubating the cells in the dark or in the light with DCMU.Cells adapted to growth on fructose showed increased rates ofrespiration both in the dark and in the light. The rate of 14CO2evolved from radiolabelled fructose was lower in the light thanin the dark or in the presence of DCMU. This fact can be partiallyexplained by the photosynthetic reutilization of respiratory14CO2. Modifications in photosynthesis were observed in fructose-growncells. PS I and PS II activity measured in spheroplasts obtainedby lysozyme treatment were enhanced by fructose probably asa way to compensate the lower concentration of chorophyll showedby fructose-grown cells. The photosynthetic affinity for externalCO2 and the rate of photosynthesis dependent on external inorganiccarbon were reduced by fructose. (Received September 24, 1991; Accepted February 14, 1992) |
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