Down-regulation of human cytomegalovirus UL138, a novel latency-associated determinant, by hcmv-miR-UL36 |
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Authors: | Yujing Huang Ying Qi Yanping Ma Rong He Yaohua Ji Zhengrong Sun Qiang Ruan |
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Institution: | 2. Clinical Genetics Department, the Affiliated Shengjing Hospital, China Medical University, 110004, Shenyang, Liaoning, P.R. China 1. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004, Shenyang, Liaoning, P.R. China
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Abstract: | MicroRNAs (miRNAs) are small RNAs, 19–23 nucleotides in length, which regulate a variety of cellular processes. Human cytomegalovirus (HCMV) encodes only one intronic miRNA: human cytomegalovirus microRNA UL36 (hcmv-miR-UL36). In this study, we found that over-expression of hcmv-miR-UL36 resulted in a more than threefold increase in HCMV DNA synthesis at 24 h post infection. Fifteen putative targets of hcmv-miR-UL36 were identified using hybrid PCR, one being the HCMV UL138 gene that has previously been identified as a novel latency-associated determinant of HCMV infection. Down-regulation of UL138 expression by hcmv-miR-UL36 was validated using luciferase reporter assays and Western blot analysis in HEK293 cells. In the presence of hcmv-miR-UL36, we observed a 74.6% decrease in luciferase activity and a 46.2% decrease in HCMV UL138 protein expression. Our results indicate that hcmv-miR-UL36 may be a viral miRNA contributing to HCMV replication. |
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