Automatic antifungal activity analyzing system on the basis of dynamic growth process of a single hypha |
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| Authors: | Satoru Yamada Jinan Cao Osao Sumita Kikuo Kurasawa Hiroshi Kurata Kibong Oh Hideaki Matsuoka |
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| Institution: | (1) Research and Development Division, Bio-Giken Inc., 1-5-8, Iwamotocho, Chiyoda-ku, 101 Tokyo, Japan;(2) The Tokyo Kembikyoin Foundation, Kudanminami, Chiyodaku, 101 Tokyo, Japan;(3) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16,Nakamachi, Koganei, 184 Tokyo, Japan |
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| Abstract: | A system for the evaluation of antifungal activity of volatile compounds has been developed that is based on dynamic growth of a single hypha. The newly developed system is composed of a reaction vessel under a microscope, automatic stage, charge coupled device (CCD) camera, TV monitor, video tape recorder (VTR), and a microcomputer. A fungus was inoculated in the reaction vessel containing agar medium and then was treated with an antifungal reagent in the gas phase either in batch or flow reaction manner. The apex of a growing hypha displayed on a TV monitor was followed automatically. From the ratio of the growth rate under exposure of a reagent (UEXPO) to the growth rate before the exposure (UPRE), the antifungal activity was expressed quantitatively. |
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| Keywords: | Antifungal activity automatic analyzer dynamic growth process single hypha |
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