Cloning of randomly sheared DNA fragments from a phi 105 lysogen of Bacillus subtilis identification of prophage-containing clones |
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| Authors: | K W Hutchison H O Halvorson |
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| Institution: | Rosenstiel Basic Medical Sciences Research Center, Brande is University, Waltham, MA 02254 U.S.A. |
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| Abstract: | A gene bank for Bacillus subtilis has been developed by cloning randomly sheared DNA fragments of a B. subtilis (phi 105) lysogen DNA in Escherichia coli employing the pMB9 plasmid vector. The DNA was inserted by the oligo(dA)-oligo(dT) method, and the average insert size of the cloned DNA was 7 kilobase pairs (kb). Three clones have been identified which carry DNA from the phi 105 prophage. None of these clones contain the phage-chromosome junction. |
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| Keywords: | Gene bank recombinant DNA pMB9 plasmid vector poly(dA)-poly(dT) tailing AT adenine-thymine kb kilobase pairs |
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