Cryo-electron tomography of the magnetotactic vibrio Magnetovibrio blakemorei: Insights into the biomineralization of prismatic magnetosomes |
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| Authors: | Fernanda Abreu Alioscka A Sousa Maria A Aronova Youngchan Kim Daniel Cox Richard D Leapman Leonardo R Andrade Bechara Kachar Dennis A Bazylinski Ulysses Lins |
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| Institution: | 1. Instituto de Microbiologia Paulo de Góes, CCS, Bloco I, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil;2. National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA;3. Center for Computational Materials Science, Naval Research Laboratory, Washington, DC 20375, USA;4. Instituto de Ciências Biomédicas, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil;5. Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA;6. School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, NV 89154, USA |
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| Abstract: | We examined the structure and biomineralization of prismatic magnetosomes in the magnetotactic marine vibrio Magnetovibrio blakemorei strain MV-1 and a non-magnetotactic mutant derived from it, using a combination of cryo-electron tomography and freeze-fracture. The vesicles enveloping the Magnetovibrio magnetosomes were elongated and detached from the cell membrane. Magnetosome crystal formation appeared to be initiated at a nucleation site on the membrane inner surface. Interestingly, while scattered filaments were observed in the surrounding cytoplasm, their association with the magnetosome chains could not be unequivocally established. Our data suggest fundamental differences between prismatic and octahedral magnetosomes in their mechanisms of nucleation and crystal growth as well as in their structural relationships with the cytoplasm and plasma membrane. |
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