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Screening a Peptide Library by DSC and SAXD: Comparison with the Biological Function of the Parent Proteins
Authors:Ana J. Pérez-Berná   George Pabst  Peter Laggner  José Villalaín
Affiliation:1. Instituto de Biología Molecular y Celular, Universidad “Miguel Hernández”, Alicante, Spain.; 2. Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Graz, Austria.;University of Minnesota, United States of America
Abstract:We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the Lβ-Lα and Lα-HII phospholipid phase transitions as well as check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding.
Keywords:
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