Carbohydrate antigens and lectin receptors of the plasma membrane of carrot cells |
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Authors: | J P Knox K Roberts |
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Institution: | (1) Department of Cell Biology, John Innes Institute, Norwich |
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Abstract: | Summary A monoclonal antibody (JIM 1) has been derived, subsequent to immunization of rats with carrot protoplasts and a hybridoma screen of protoplast immunoagglutination, that recognizes a determinant at the outer face of the plasma membrane of carrot cells. The binding of JIM 1 is readily inhibitable by -D-galactosyl residues. Although weakly cross-reacting with an extracellular arabinogalactan protein, isolated from the conditioned medium of suspension-cultured carrot cells, JIM 1 does not recognize arabinogalactan proteins associated with the plasma membrane. The plasma membrane antigen recognized by JIM 1 was of low molecular weight and was sensitive to both periodate treatment and a protease. JIM 1 therefore defines a new class of galactosyl-residue containing plant cell surface antigen, distinct from the arabinogalactan proteins. However, the extracellular arabinogalactan protein and related plasma membrane-associated glycoproteins are demonstrated to bind the anti-galactose plant lectin peanut agglutinin.Abbrevations AGP
arabinogalactan protein
- McAb
monoclonal antibody
- PNA
peanut agglutinin |
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Keywords: | Antigenic determinants Arabinogalactan proteins Lectin Plasma membrane Daucus carota |
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