Gradient acrylamide/agarose gels for electrophoretic separation of intact human very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins |
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| Authors: | L T Lee M Lefevre L Wong P S Roheim J J Thompson |
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| Affiliation: | 1. Department of Physiology, Louisiana State University Medical Center, New Orleans, Louisiana 70112 USA;2. Department of Microbiology and Immunology, Louisiana State University Medical Center, New Orleans, Louisiana 70112 USA;1. Graduate School of Arts and Sciences, the University of Tokyo, Tokyo 153-8505, Japan;2. Graduate School of Information Science and Technology, the University of Tokyo, Tokyo 113-8656, Japan;3. Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan;1. Department of Materials Science and Engineering, Institute of Glass and Ceramics (WW3), Friedrich-Alexander University Erlangen-Nürnberg (FAU), Martensstrasse 5, 91058 Erlangen, Germany;2. Interdisciplinary Center for Functional Particle Systems (FPS), Friedrich-Alexander University Erlangen-Nürnberg (FAU), Haberstrasse 9a, 91058 Erlangen, Germany;3. Department of Earth and Planetary Sciences, 16 University Avenue, Macquarie University, NSW 2109, Australia;4. Institute of Inorganic and Analytical Chemistry, Johannes Gutenberg-University of Mainz, Duesbergweg 10-14, Germany;5. Department of Mineralogy and Petrology, University of Granada, Spain, Fuentenueva s/n, 18071 Granada, Spain;1. Istituto per la Conservazione e la Valorizzazione dei Beni Culturali, CNR, Via Cozzi 53, 20125 Milano, Italy;2. DISAT, Università di Milano Bicocca, Milan, Italy;3. Dipartimento di Scienze della Terra, Università degli Studi di Milano, Via Botticelli 23, I-20133 Milano, Italy;4. Istituto di Cristallografia, CNR, Via G. Amendola 122/o, Bari, Italy;5. Dipartimento Chimica, Materiali e Ingegneria Chimica “Giulio Natta”, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy;1. Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201240, China;2. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China;3. Biodesign Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;4. Division of Physical Biology, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;5. Center for Proteomics and Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4988, USA |
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| Abstract: | An exponential gradient gel with 0-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins in a size-dependent fashion. The characteristic relative mobility of these species can be determined in relation to protein and colloidal gold reference materials. Electron microscopy of selected lipoprotein fractions confirmed that relative mobility was related to apparent lipoprotein diameter. The composite gel medium can be used with prestained lipoproteins and permits immunoelectroblotting for qualitative analysis of apolipoprotein constituents. |
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