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Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy |
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| Authors: | Li Mi Liu Lianqing Xi Ning Wang Yuechao Dong Zaili Tabata Osamu Xiao Xiubin Zhang Weijing |
| Affiliation: | aState Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China;bGraduate University of Chinese Academy of Sciences, Beijing 100049, China;cDepartment of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA;dDepartment of Micro Engineering, Kyoto University, Kyoto 606-8501, Japan;eDepartment of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China |
| Abstract: | The topography and mechanical properties of single B-lymphoma cells have been investigated by atomic force microscopy (AFM). With the assistance of microfabricated patterned pillars, the surface topography and ultrastructure of single living B-lymphoma cell were visualized by AFM. The apoptosis of B-lymphoma cells induced by rituximab alone was observed by acridine orange/ethidium bromide (AO/EB) double fluorescent staining. The rituximab-induced changes of mechanical properties in B-lymphoma cells were measured dynamically and the results showed that B-lymphoma cells became dramatically softer after incubation with rituximab. These results can improve our understanding of rituximab’effect and will facilitate the further investigation of the underlying mechanisms. |
| Keywords: | Atomic force microscopy Mechanical properties Rituximab Young&rsquo s modulus |
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