| Effect of Human WEE1 and Stem Cell Factor on Human CD34^＋ Umbilical Cord Blood Cell Damage Induced by Chemotherapeutic Agents |
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| 作者姓名： | LEI P HE Y SHI W PENG J WU S ZHU H CHEN J SHEN G |
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| 作者单位： | [1]Laboratory of Molecular and lmmuno-Pharmacology, Department of Immunology and Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China [3]Department of Immunology, Yunyang Medical College, Shiyan 442000, China |
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| 基金项目： | This work was supported by the grants from the National Natural Science Foundation of China （No. 30170879） and the Key Program of Education Department of Hubei Province （No. 2003A008） |
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| 摘 要： | Myelosuppression is one of the major side-effects of most anticancer drugs. To achieve myeloprotection, one bicistronic vector encoding anti-apoptotic protein human WEE l （WEElHu） and proliferation-stimulating stem cell factor （SCF） was generated. In this study, we selected human umbilical cord blood CD34^＋ cells as the in vitro model in an attempt to investigate whether WEEIHu, rather than conventional drug-resistant genes, can be introduced to rescue cells from the damage by chemotherapeutic agents such as cisplatin, adriamycin, mitomycin-c and 5-fluorouracil. Cell viability and cytotoxicity assay, colony-forming units in culture assay and externalization of phospholipid phosphatidylserine analysis showed that the expression of WEElHu and SCF in CD34^＋ cells provided the cells with some protection. These findings suggest that the expression of WEElHu and SCF might rescue CD34^＋ cells from chemotherapyinduced myelosuppression.
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| 关 键 词： | 放射化疗法 融合基因构建 干细胞因子 骨髓抑制 |
| 修稿时间： | 2007-02-06 |
Effect of human WEE1 and stem cell factor on human CD34+ umbilical cord blood cell damage induced by chemotherapeutic agents |
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| LEI P,HE Y,SHI W,PENG J,WU S,ZHU H,CHEN J,SHEN G.Effect of human WEE1 and stem cell factor on human CD34+ umbilical cord blood cell damage induced by chemotherapeutic agents[J].Acta Biochimica et Biophysica Sinica,2007,39(8):599-607. |
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| Authors: | LEI Ping HE Yong SHI Wenfang PENG Jilin WU Sha ZHU Huifen CHEN Jianguo SHEN Guanxin |
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| Institution: | Laboratory of Molecular and Immuno-Pharmacology, Department of Immunology and Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
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| Abstract: | Myelosuppression is one of the major side-effects of most anticancer drugs. To achieve myeloprotection, one bicistronic vector encoding anti-apoptotic protein human WEE1 (WEE1Hu) and proliferation-stimulating stem cell factor (SCF) was generated. In this study, we selected human umbilical cord blood CD34+ cells as the in vitro model in an attempt to investigate whether WEE1Hu, rather than conventional drug-resistant genes, can be introduced to rescue cells from the damage by chemotherapeutic agents such as cisplatin, adriamycin, mitomycin-c and 5-fluorouracil. Cell viability and cytotoxicity assay, colony-forming units in culture assay and externalization of phospholipid phosphatidylserine analysis showed that the expression of WEE1Hu and SCF in CD34+ cells provided the cells with some protection. These findings suggest that the expression of WEE1Hu and SCF might rescue CD34+ cells from chemotherapy-induced myelosuppression. |
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| Keywords: | WEEl Hu SCF myelosuppression radiochemotherapy |
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