Estimation of nuclear DNA content of various bamboo and rattan species |
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| Authors: | Prakash P Kumar Ian M Turner A Nagaraja Rao K Arumuganathan |
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| Institution: | (1) Department of Biological Sciences, National University of Singapore, 10 Science Drive 4, Singapore, 117543, Singapore;(2) Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore;(3) Present address: P.O. Box 20, Winchelsea, TN36 4WA, UK;(4) 131 Cavenagh Road, #12-01, Singapore, 229627, Singapore;(5) Flow Cytometry and Imaging Core Laboratory, Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA |
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| Abstract: | We determined the nuclear DNA content (genome size) of over 35 accessions each of bamboo and rattan species from Southeast
Asia. The 2C DNA per nucleus was quantified by flow cytometry. The fluorescence of nuclei isolated from the leaves and stained
with propidium iodide was measured. The genome size of the bamboo species examined was between 2.5 and 5.9 pg DNA per 2C nucleus.
The genome size of the rattan species examined ranged from 1.8 to 10.5 pg DNA per 2C nucleus. This information will be useful
for scientists working in diverse areas of plant biology such as biotechnology, biodiversity, genome analysis, plant breeding,
physiology and molecular biology. Such data may be utilized to attempt to correlate the genome size with the ploidy status
of bamboo species in cases where ploidy status has been reported. |
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