Production and secretion of a heterologous protein by turnip hairy roots with superiority over tobacco hairy roots |
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Authors: | Yoann Huet Jean-Pierre Ele Ekouna Aurore Caron Katiba Mezreb Michèle Boitel-Conti François Guerineau |
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Institution: | 1. Biologie des Plantes et Innovation (BioPI), Université de Picardie Jules Verne, 33 rue St Leu, 80039, Amiens, France 2. Root Lines Technology, CRRBM, 33 rue St Leu, 80039, Amiens, France
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Abstract: | A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaMV polyadenylation signal were constructed. One cassette was used to express the green fluorescent protein (GFP)-encoding gene in hairy roots grown in flasks. A stable and fast-growing hairy root line secreted GFP at >120 mg/l culture medium. GFP represented 60 % of the total soluble proteins in the culture medium. Turnip hairy roots retained sustainable growth and stable GFP production over 3 years. These results were superior to those obtained using tobacco hairy roots. |
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