蓝粒小麦易位系的荧光原位杂交鉴定

普通小麦（Triticum aestivum L.)和长穗偃麦草（Agropyron elongatum (Host)Beauv=Elytriga elongatum(Host)Nevski=Thinopyrum ponticum (Host)Barkworth and Dewey,2n=10x=70)杂交后选育出的蓝粒小麦异代换系（蓝５８），２n=42其中９９０６中被易位蓝粒片段的相对长度约占易位小麦染色体短臂的１／３，而９９０２中被易位蓝粒片段的相对长度约占易位小麦染色体长臂的１／２，并将９９０２的蓝粒易位片段定位在小麦Ｄ组染色体上；（２）９９１５易位附加和９９０４易位－易位附加，其体细胞染色体数均为４４，其中９９１５的体细胞染色体只有一对发生了易位，另外队了两条长穗偃麦草染色体；而９９０４有两对染色体发生了易位，并易位系中控制蓝粒性状的长穗偃麦草染色体片段的定位和蓝粒小麦易位系的应用进行了讨论。

Identification of Blue-grained Wheat Translocation Lines Using Fluorescence in Situ Hybridization

The blue_grained wheat substitution line (blue 58) originated from wild hybridization between Triticum aestivum L. and Agropyron elongatum (Host) Beauv= Elytrigia elongatum (Host) Nevski= Thinopyrum ponticum (Host) Barkworth and Dewey (2n=10x=70) was irradiated and four translocation lines were screened by fluorescence in situ hybridization from the offsprings. The results obtained include the following: (1) both the two translocation lines, 9906 and 9902, have 42 chromosomes. The length of the translocated blue_grained segment was approximately one_third of the short_arm and one_half of the long_arm of the translocated wheat chromosome in 9906 and 9902, respectively, and the blue_grained translocated segment in 9902 was located on D genome; (2) both 9915 and 9904 have 44 chromosomes. One pair of chromosomes was translocated and two chromosomes from Th. ponticum were added in 9903, while two pairs of chromosomes were translocated in 9904 by blue_grained wheat segment. The location and application of blue_grained wheat translocation lines were discussed.